Difference between revisions of "Part:BBa K2088017"

 
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This part includes optimized mhbH (using Codon Optimization Tool of IDT) and a designed rbs (using RBS Calculator).  
 
This part includes optimized mhbH (using Codon Optimization Tool of IDT) and a designed rbs (using RBS Calculator).  
−
mhbH encodes Fumarylpyruvate hydrolase.
+
mhbH encodes Fumarylpyruvate hydrolase.We choose Escherichia coli K12 as the organism to optimize the sequences.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 10:49, 11 October 2016


J23100+B0034+BBa_K2088005

This part includes optimized mhbH (using Codon Optimization Tool of IDT) and a designed rbs (using RBS Calculator). mhbH encodes Fumarylpyruvate hydrolase.We choose Escherichia coli K12 as the organism to optimize the sequences.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 619
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 92
  • 1000
    COMPATIBLE WITH RFC[1000]