Difference between revisions of "Part:BBa K2148008"
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This is a coding DNA sequence (CDS) for a yellow fluorescent protein which is codon-optimised for Chlamydomonas reinhardtii chloroplast. It is coded as a CDS/CTAG Phytobrick. | This is a coding DNA sequence (CDS) for a yellow fluorescent protein which is codon-optimised for Chlamydomonas reinhardtii chloroplast. It is coded as a CDS/CTAG Phytobrick. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
+ | This is a level-0 Phytobrick conforming to the Phytobrick standard. It is coded as a CDS/CTAG Phytobrick and can be used with other level-0 Phytobricks to create a transcriptional unit that expresses a yellow Venus fluorescent protein for Chlamydomonas reinhardtii. | ||
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<partinfo>BBa_K2148008 parameters</partinfo> | <partinfo>BBa_K2148008 parameters</partinfo> | ||
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+ | ===Characterisation=== | ||
+ | |||
+ | ===References=== | ||
+ | Rasala, B. A., Barrera, D. J., Ng, J., Plucinak, T. M., Rosenberg, J. N., Weeks, D. P., Oyler, G. A., Peterson, T. C., Haerizadeh, F. and Mayfield, S. P. (2013), Expanding the spectral palette of fluorescent proteins for the green microalga Chlamydomonas reinhardtii. Plant J, 74: 545–556. doi:10.1111/tpj.12165 |
Latest revision as of 09:43, 11 October 2016
mVenus C.reinhardtii-CO Yellow FP
This is a coding DNA sequence (CDS) for a yellow fluorescent protein which is codon-optimised for Chlamydomonas reinhardtii chloroplast. It is coded as a CDS/CTAG Phytobrick.
Usage and Biology
This is a level-0 Phytobrick conforming to the Phytobrick standard. It is coded as a CDS/CTAG Phytobrick and can be used with other level-0 Phytobricks to create a transcriptional unit that expresses a yellow Venus fluorescent protein for Chlamydomonas reinhardtii.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 668
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 668
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 668
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 668
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 668
- 1000COMPATIBLE WITH RFC[1000]
Characterisation
References
Rasala, B. A., Barrera, D. J., Ng, J., Plucinak, T. M., Rosenberg, J. N., Weeks, D. P., Oyler, G. A., Peterson, T. C., Haerizadeh, F. and Mayfield, S. P. (2013), Expanding the spectral palette of fluorescent proteins for the green microalga Chlamydomonas reinhardtii. Plant J, 74: 545–556. doi:10.1111/tpj.12165