Difference between revisions of "Part:BBa K1926011:Design"
(→‎Design Notes)
Line 6: Line 6:
  
 
===Design Notes===
 
===Design Notes===
−
We got this sequence from human genome through PCR using the following primers:
 
  
−
pCCNE-F: CGTGTTTACATTCCACCCGCGCCA
+
The SNAP-tag® with tags and RTSs are constructed using PCR and oligonucleotide ligation by the following procedure. Our UNITs all contain XbaI and SpeI enzyme sites, so we can easily line up the UNITs using 3A assembly. 
  
−
pCCNE-R: TGATGGGGCTGCTCCGGCCT
+
[[Image:T--SYSU-CHINA--result-img.jpeg|800px|thumb|left|'''Figure 1:''' Three steps to construct our UNITs using PCR and oligonucleotide ligation.]]
−
 
+
<br style="clear: both" />
−
(Product: 986)
+
  
 
===Source===
 
===Source===
  
 
The sequence was retrieved from Addgene.
 
The sequence was retrieved from Addgene.

Revision as of 08:29, 11 October 2016

The SNAP UNIT: SNAP-tag flanked by loxP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 419
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The SNAP-tag® with tags and RTSs are constructed using PCR and oligonucleotide ligation by the following procedure. Our UNITs all contain XbaI and SpeI enzyme sites, so we can easily line up the UNITs using 3A assembly.

Figure 1: Three steps to construct our UNITs using PCR and oligonucleotide ligation.


Source

The sequence was retrieved from Addgene.