Difference between revisions of "Part:BBa K1926002:Design"

(→‎References)
(→‎Design Notes)
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
We got it from human genome through PCR using the following primers:
+
We got the sequence from human genome through PCR using the following primers:
  
 
pKi67-F: ACCTCTGCCCTCCGCCAGCCG
 
pKi67-F: ACCTCTGCCCTCCGCCAGCCG

Revision as of 04:16, 11 October 2016


A cyclic promoter of Ki-67 from human genome


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 425
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 422


Design Notes

We got the sequence from human genome through PCR using the following primers:

pKi67-F: ACCTCTGCCCTCCGCCAGCCG

pKi67-R: ACCCGGTGGCCCTACAGGCTACG

(Product: 360)

Source

The sequence was retrieved from Addgene.

References

1. Pei, D.S., et al., Analysis of human Ki-67 gene promoter and identification of the Sp1 binding sites for Ki-67 transcription. Tumour Biol, 2012. 33(1): p. 257-66.