Difference between revisions of "Part:BBa K1926002:Design"

 
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
−
Mutation to remove Pst1
+
We got it from human genome through PCR using the following primers:
  
 +
pKi67-F: ACCTCTGCCCTCCGCCAGCCG
  
 +
pKi67-R: ACCCGGTGGCCCTACAGGCTACG
 +
 +
(Product: 360)
  
 
===Source===
 
===Source===

Revision as of 03:57, 11 October 2016


A cyclic promoter of Ki-67 from human genome


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 425
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 422


Design Notes

We got it from human genome through PCR using the following primers:

pKi67-F: ACCTCTGCCCTCCGCCAGCCG

pKi67-R: ACCCGGTGGCCCTACAGGCTACG

(Product: 360)

Source

The sequence was retrieved from Addgene. We got it from human genome through PCR using the following primers: pKi67-F: ACCTCTGCCCTCCGCCAGCCG pKi67-R: ACCCGGTGGCCCTACAGGCTACG (Product: 360)


References