Difference between revisions of "Part:BBa K1897005"

Revision as of 21:10, 10 October 2016

HasI sigma factor coding sequence

Usage and Biology

HasI ia a 19 kDa protein that acts as an extra cytoplasmic function sigma factor under the Has operon system. The Has system is a heme acquisition system originally from the Serratia marcescens. This cytoplasmic protein is usually bound to the anti-sigma factor HasS and therefore is inactive. When the holo-HasA hemophore binds to the cell surface receptor HasR, it triggers a conformational change in HasS. This then releases the HasI which will then trigger expression of genes under the control of the Has promoter.

Characterisation of this part is done in tandem with the full has operon (BBa_K1897007)

References

Biville, F., Cwerman, H., Létoffé, S., Rossi, M. S., Drouet, V., Ghigo, J. M., & Wandersman, C. (2004). Haemophore‐mediated signalling in Serratia marcescens: a new mode of regulation for an extra cytoplasmic function (ECF) sigma factor involved in haem acquisition. Molecular microbiology, 53(4), 1267-1277.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NotI site found at 42
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 ===Usage and Biology===
-HasI ia a 19 kDa protein that acts as an extracellular sigma factor under the Has operon system. The Has system is a heme acquisition system originally from the ''Serratia marcescens''. This cytoplasmic protein is usually bound to the anti-sigma factor [https://parts.igem.org/Part:BBa_K1897003 HasS] and therefore is inactive. When the [https://parts.igem.org/Part:BBa_K1897001 holo-HasA hemophore] binds to the cell surface receptor [https://parts.igem.org/Part:BBa_K1897002 HasR], it triggers a conformational change in [https://parts.igem.org/Part:BBa_K1897003 HasS]. This then releases the HasI which will then trigger expression of genes under the control of the [https://parts.igem.org/Part:BBa_K1897006 Has promoter].
+HasI ia a 19 kDa protein that acts as an extra cytoplasmic function sigma factor under the Has operon system. The Has system is a heme acquisition system originally from the ''Serratia marcescens''. This cytoplasmic protein is usually bound to the anti-sigma factor [https://parts.igem.org/Part:BBa_K1897003 HasS] and therefore is inactive. When the [https://parts.igem.org/Part:BBa_K1897001 holo-HasA hemophore] binds to the cell surface receptor [https://parts.igem.org/Part:BBa_K1897002 HasR], it triggers a conformational change in [https://parts.igem.org/Part:BBa_K1897003 HasS]. This then releases the HasI which will then trigger expression of genes under the control of the [https://parts.igem.org/Part:BBa_K1897006 Has promoter].
 Characterisation of this part is done in tandem with the full has operon ([https://parts.igem.org/Part:BBa_K1897007 BBa_K1897007])