Difference between revisions of "Part:BBa K1897003"

 
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<partinfo>BBa_K1897003 short</partinfo>
 
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HasR is the membrane-bound receptor in the Has system, a heme acquisition system originally from the Serratia marcescens. The HasR protein is involved in binding with a heme-loaded HasA hemophore to trigger downstream HasS and HasI. It has been shown that the N terminal of HasR is especially crucial for signal transduction. With the heme-loaded hasA bound to hasR, it induces a conformational change in HasR that causes the activation of HasS, an anti-sigma factor. HasS then releases HasI (sigma factor) that was initially bound to it and HasI ultimately is able to trigger expression of genes under control of pHas (part number).
 
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===Usage and Biology===
 
===Usage and Biology===
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HasS (34.7 kDa) is the anti-sigma factor in the Has system signalling cascade. The Has system is a heme acquisition system originally from the ''Serratia marcescens''. HasS is responsible for binding to the cytoplasmic extracellular sigma factor, [https://parts.igem.org/Part:BBa_K1897005 HasI], inhibiting its activity. When the [https://parts.igem.org/Part:BBa_K1897001 holo-HasA hemophore] binds to the cell surface receptor [https://parts.igem.org/Part:BBa_K1897003 HasR], it triggers a conformational change in HasS. This then releases the HasI which binds to the [https://parts.igem.org/Part:BBa_K1897006 Has promoter]. This will allow for the expression of genes controlled by that promoter.
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Characterisation of this part is done in tandem with the full has operon ([https://parts.igem.org/Part:BBa_K1897007 BBa_K1897007])
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1897003 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1897003 SequenceAndFeatures</partinfo>
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===Functional Parameters===
 
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<partinfo>BBa_K1897003 parameters</partinfo>
 
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Revision as of 19:01, 10 October 2016


HasS antisigma factor coding sequence

Usage and Biology

HasS (34.7 kDa) is the anti-sigma factor in the Has system signalling cascade. The Has system is a heme acquisition system originally from the Serratia marcescens. HasS is responsible for binding to the cytoplasmic extracellular sigma factor, HasI, inhibiting its activity. When the holo-HasA hemophore binds to the cell surface receptor HasR, it triggers a conformational change in HasS. This then releases the HasI which binds to the Has promoter. This will allow for the expression of genes controlled by that promoter.

Characterisation of this part is done in tandem with the full has operon (BBa_K1897007)


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 34
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 317
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 72
  • 1000
    COMPATIBLE WITH RFC[1000]