Difference between revisions of "Part:BBa K1933002"
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<partinfo>BBa_K1933002 short</partinfo> | <partinfo>BBa_K1933002 short</partinfo> | ||
− | This part codes anti-Norovirus GII.4 scFv (single-chain variable fragment) which derives from human 12A2 antibody. This scFv is suggested to prevent binding of human norovirus to Hist-Blood Group Antigens[1]. This coding sequence was fused to INPNC-His (part number: ) or BclA-His (part number: ) in our project. | + | This part codes for anti-Norovirus GII.4 scFv (single-chain variable fragment) which derives from human 12A2 antibody. This scFv is suggested to prevent binding of human norovirus to Hist-Blood Group Antigens[1]. This coding sequence was fused to INPNC-His (part number: ) or BclA-His (part number: ) in our project. |
For more information, please visit [http://2016.igem.org/Team:Kyoto our wiki] | For more information, please visit [http://2016.igem.org/Team:Kyoto our wiki] | ||
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==Reference== | ==Reference== | ||
+ | |||
+ | ==Judging== | ||
+ | |||
[1]Higo‐Moriguchi, Kyoko, et al. "Isolation of cross‐reactive human monoclonal antibodies that prevent binding of human noroviruses to histo‐blood group antigens." Journal of medical virology 86.4 (2014): 558-567. | [1]Higo‐Moriguchi, Kyoko, et al. "Isolation of cross‐reactive human monoclonal antibodies that prevent binding of human noroviruses to histo‐blood group antigens." Journal of medical virology 86.4 (2014): 558-567. |
Revision as of 18:58, 10 October 2016
anti-Norovirus GII.4 scFv
This part codes for anti-Norovirus GII.4 scFv (single-chain variable fragment) which derives from human 12A2 antibody. This scFv is suggested to prevent binding of human norovirus to Hist-Blood Group Antigens[1]. This coding sequence was fused to INPNC-His (part number: ) or BclA-His (part number: ) in our project. For more information, please visit [http://2016.igem.org/Team:Kyoto our wiki]
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 387
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Usage
Biology
Characterization
Reference
Judging
[1]Higo‐Moriguchi, Kyoko, et al. "Isolation of cross‐reactive human monoclonal antibodies that prevent binding of human noroviruses to histo‐blood group antigens." Journal of medical virology 86.4 (2014): 558-567.