Difference between revisions of "Part:BBa K1897002"

Revision as of 18:46, 10 October 2016

HasR Membrane receptor coding sequence

Usage and Biology

HasR is a 98.22 kDa membrane-bound receptor in the Has system, a heme acquisition system originally from the Serratia marcescens. The HasR protein is involved in binding with a heme-loaded holo-HasA hemophore to trigger downstream signalling events. The signal transduction fails to occur with exposure to only haem or the apo-HasA (Rossi et al., 2003). Furthermore, it has been shown that the N terminal of HasR is especially crucial for signal transduction (Biville et al., 2004). With the holo-hasA bound to HasR, it induces a conformational change in HasR that causes the activation of HasS, an anti-sigma factor. HasS then releases HasI (sigma factor) initially bound to it and HasI ultimately is able to trigger expression of genes under control of Has operon promoter (BBa_K1897006).

Characterisation of this part is done in tandem with the full has operon (BBa_K1897007)

References

Biville, F., Cwerman, H., Létoffé, S., Rossi, M. S., Drouet, V., Ghigo, J. M., & Wandersman, C. (2004). Haemophore‐mediated signalling in Serratia marcescens: a new mode of regulation for an extra cytoplasmic function (ECF) sigma factor involved in haem acquisition. Molecular microbiology, 53(4), 1267-1277.

Rossi, M. S., Paquelin, A., Ghigo, J. M., & Wandersman, C. (2003). Haemophore‐mediated signal transduction across the bacterial cell envelope in Serratia marcescens: the inducer and the transported substrate are different molecules. Molecular microbiology, 48(6), 1467-1480.

Sequence and Features