Difference between revisions of "Part:BBa K1933002"

Revision as of 18:25, 10 October 2016

anti-Norovirus GII.4 scFv

This part codes anti-Norovirus GII.4 scFv (single-chain variable fragment) which derives from human 12A2 antibody. This scFv is suggested to prevent binding of human norovirus to Hist-Blood Group Antigens[1]. This coding sequence was fused to INPNC-His (part number: ) or BclA-His (part number: ) in our project. For more information, please visit [http://2016.igem.org/Team:Kyoto our wiki]

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 387
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Usage

Biology

Characterization

Reference

[1]Higo‐Moriguchi, Kyoko, et al. "Isolation of cross‐reactive human monoclonal antibodies that prevent binding of human noroviruses to histo‐blood group antigens." Journal of medical virology 86.4 (2014): 558-567.

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 <partinfo>BBa_K1933002 short</partinfo>
-This part codes anti-Norovirus GII.4 scFv (single-chain variable fragment) which derives from human 12A2 antibody. This scFv is suggested to prevent binding of human norovirus to Hist-Blood Group Antigens. [1] This coding sequence was fused to INPNC-His (part number: ) or BclA-His (part number: ) in our project.
+This part codes anti-Norovirus GII.4 scFv (single-chain variable fragment) which derives from human 12A2 antibody. This scFv is suggested to prevent binding of human norovirus to Hist-Blood Group Antigens[1]. This coding sequence was fused to INPNC-His (part number: ) or BclA-His (part number: ) in our project.
 For more information, please visit [http://2016.igem.org/Team:Kyoto our wiki]