Difference between revisions of "Part:BBa J100306"

Latest revision as of 17:49, 10 October 2016

repClone Red (J100205) with wt TetR promoter (R0040)

repClone Red is a construct we designed to allow students and researchers to investigate the TetR repressible promoter. The RFP reporter system in repClone provides phenotypic evidence of the interference of anhydrous tetracycline with repression of the TetR promoter. This construct uses the wild type TetR promoter sequence. Researchers can manipulate this sequence by mutating, inserting, or deleting bases. They can then compare RFP expression in their new constructs in the presence and absence of aTc to test the effects on the TetR repressor system. This will allow researchers and biology students to explore sequence requirements for the interactions between the TetR repressible promoter, TetR protein, and aTc.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal SpeI site found at 9
12
INCOMPATIBLE WITH RFC[12]
Illegal SpeI site found at 9
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal SpeI site found at 9
25
INCOMPATIBLE WITH RFC[25]
Illegal SpeI site found at 9
Illegal AgeI site found at 2213
Illegal AgeI site found at 2325
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_J100306 short</partinfo>
-repClone Red is a construct we designed to allow students and researchers to investigate the TetR repressible promoter. The RFP reporter system in repClone provides phenotypic evidence of the interference of anhydrous tetracycline with repression of the TetR promoter. This construct uses the wild type TetR promoter sequence. Researchers can manipulate this sequence by mutating, inserting, or deleting bases. They can then compare RFP expression in their new constructs in the presence and absence of aTc to test the effects on the TetR repressor system. This will allow researchers and biology students to discover which regions of the promoter are essential for promoting transcription and which are involved in repression.
+repClone Red is a construct we designed to allow students and researchers to investigate the TetR repressible promoter. The RFP reporter system in repClone provides phenotypic evidence of the interference of anhydrous tetracycline with repression of the TetR promoter. This construct uses the wild type TetR promoter sequence. Researchers can manipulate this sequence by mutating, inserting, or deleting bases. They can then compare RFP expression in their new constructs in the presence and absence of aTc to test the effects on the TetR repressor system. This will allow researchers and biology students to explore sequence requirements for the interactions between the TetR repressible promoter, TetR protein, and aTc.
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