Difference between revisions of "Part:BBa K1897008:Design"
Shihuiangle (Talk | contribs) (→Design Notes) |
Shihuiangle (Talk | contribs) (→Design Notes) |
||
| Line 7: | Line 7: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | There is one HA tag available for characterisation of the protein produced via western blot. Note that the stop codon for LuxR <partinfo>BBa_C0062</partinfo> is shifted to after the HA tag. Also, the transcriptional terminators rrnBT1 (from <partinfo>BBa_B0010</partinfo>)+ <partinfo>BBa_B0012</partinfo> was derived from <partinfo>BBa_B0015</partinfo>, with the first 8 base pairs removed. | + | There is one HA tag available for characterisation of the protein produced via western blot. Note that the stop codon for LuxR <partinfo>BBa_C0062</partinfo> is shifted to after the HA tag. Also, the transcriptional terminators rrnBT1 (from <partinfo>BBa_B0010</partinfo>) + <partinfo>BBa_B0012</partinfo> was derived from <partinfo>BBa_B0015</partinfo>, with the first 8 base pairs removed. |
===Source=== | ===Source=== | ||
Latest revision as of 17:20, 10 October 2016
LuxR
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 50
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
There is one HA tag available for characterisation of the protein produced via western blot. Note that the stop codon for LuxR BBa_C0062 is shifted to after the HA tag. Also, the transcriptional terminators rrnBT1 (from BBa_B0010) + BBa_B0012 was derived from BBa_B0015, with the first 8 base pairs removed.
Source
LuxR was obtained from biobrick part BBa_C0062, which was derived from Vibrio fischeri. The promoter was synthesized based on the sequence obtained from BBa_J23119. The ribosome binding site (RBS) was synthesized based on the sequence obtained from BBa_B0030. The transcription terminators were synthesized based on the sequence obtained from BBa_B0015.
References
Shadel, G. S., & Baldwin, T. O. (1991). The Vibrio fischeri LuxR protein is capable of bidirectional stimulation of transcription and both positive and negative regulation of the luxR gene. Journal of bacteriology, 173(2), 568-574.
Trott, A. E. (2000). Amino Acid Residues in LuxR Critical for its Mechanism of Transcriptional Activation during Quorum Sensing (Doctoral dissertation, Virginia Polytechnic Institute and State University).