Difference between revisions of "Part:BBa K1897008:Design"

Revision as of 16:00, 10 October 2016

LuxR

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 50
1000
COMPATIBLE WITH RFC[1000]

Design Notes

There is one HA tag available for characterisation of the protein produced via western blot. Also, the terminator rrnBT1+BBa_B0012 was derived from BBa_B0015, with the first 8 base pairs removed.

Source

LuxR was obtained from biobrick part BBa_C0062, which was derived from Vibrio fischeri. The promoter was synthesized based on the sequence obtained from BBa_J23119. The ribosome binding site (RBS) was synthesized based on the sequence obtained from BBa_B0030. The transcription terminators were synthesized based on the sequence obtained from BBa_B0015.

References

Shadel, G. S., & Baldwin, T. O. (1991). The Vibrio fischeri LuxR protein is capable of bidirectional stimulation of transcription and both positive and negative regulation of the luxR gene. Journal of bacteriology, 173(2), 568-574.

Trott, A. E. (2000). Amino Acid Residues in LuxR Critical for its Mechanism of Transcriptional Activation during Quorum Sensing (Doctoral dissertation, Virginia Polytechnic Institute and State University).

@@ Line 12: / Line 12: @@
 ===Source===
+LuxR was obtained from biobrick part <partinfo>BBa_C0062</partinfo>, which was derived from <i>Vibrio fischeri</i>.
-This part was made based on the genome of <i>Vibrio fischeri</i>.
+The promoter was synthesized based on the sequence obtained from <partinfo>BBa_J23119</partinfo>.
+The ribosome binding site (RBS) was synthesized based on the sequence obtained from <partinfo>BBa_B0030</partinfo>.
+The transcription terminators were synthesized based on the sequence obtained from <partinfo>BBa_B0015</partinfo>.
 ===References===