Difference between revisions of "Part:BBa K1897008"

Revision as of 15:36, 10 October 2016

LuxR

This part was made up of promoter BBa_J23119, RBS BBa_B0030, LuxR coding sequence BBa_C0062 and double terminator BBa_B0015.

This part allows for the constitutive expression of LuxR protein from the promoter BBa_J23119. The LuxR protein originated from Vibrio fischeri, and is a regulatory protein of the Lux operon. The Lux operon can be transcribed from from te left (operonL) or right (operonR). Transcription of operonR, which consists of LuxICDABE, occurs only in the presence of N-(3-oxo-hexanoyl)-homoserine lactone (AHL), which is an autoinducer produced by LuxI (Shaldel and Baldwin, 1991). This also produces light due to the production of LuxCDABE (Shaldel and Baldwin, 1991). Transcription of operonL leads to the production of LuxR (Shaldel and Baldwin, 1991). In the presence of rising amounts of AHL, LuxR-AHL complexes begin to form, and LuxR undergoes a conformational change that leads to the activation of the Lux operon (Trott, 2000).

This part can be used if you need constitutive production of LuxR to activate production of genes under the control of pLuxR (BBa_R0062).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 50
1000
COMPATIBLE WITH RFC[1000]

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 This part was made up of promoter BBa_J23119, RBS BBa_B0030, LuxR coding sequence BBa_C0062 and double terminator BBa_B0015.
-This part allows for the constitutive expression of LuxR protein from the promoter BBa_J23119. The LuxR protein originated from Vibrio fischeri, and is a regulatory protein of the Lux operon. The Lux operon can be transcribed from from te left (operonL) or right (operonR). Transcription of operonR, which consists of LuxICDABE, occurs only in the presence of N-(3-oxo-hexanoyl)-homoserine lactone (AHL), which is an autoinducer produced by LuxI. This also produces light due to the production of LuxCDABE. Transcription of operonL leads to the production of LuxR. In the presence of rising amounts of AHL, LuxR-AHL complexes begin to form, and LuxR undergoes a conformational change that leads to the activation of the Lux operon.
+This part allows for the constitutive expression of LuxR protein from the promoter BBa_J23119. The LuxR protein originated from Vibrio fischeri, and is a regulatory protein of the Lux operon. The Lux operon can be transcribed from from te left (operonL) or right (operonR). Transcription of operonR, which consists of LuxICDABE, occurs only in the presence of N-(3-oxo-hexanoyl)-homoserine lactone (AHL), which is an autoinducer produced by LuxI (Shaldel and Baldwin, 1991). This also produces light due to the production of LuxCDABE (Shaldel and Baldwin, 1991). Transcription of operonL leads to the production of LuxR (Shaldel and Baldwin, 1991). In the presence of rising amounts of AHL, LuxR-AHL complexes begin to form, and LuxR undergoes a conformational change that leads to the activation of the Lux operon (Trott, 2000).
-This part can be used if you need constitutive production of LuxR to activate production of genes under the control of pLuxR (BBa_R0062). It also consists of an enzyme cut site XbaI after the biobricks prefix and a KpnI site before the biobricks suffix.
+This part can be used if you need constitutive production of LuxR to activate production of genes under the control of pLuxR (BBa_R0062).
 <!-- Add more about the biology of this part here