Difference between revisions of "Part:BBa K2110008"
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<span class='h3bb'>Reference</span>
 
<span class='h3bb'>Reference</span>
  
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Previous part BBa_K339007 acted as our reference, we improved its application by changing the mRFP gene to the ddpX gene.
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Previous part <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K339007"_blank">BBa_K339007 </a>acted as our reference, we improved its application by changing the mRFP gene to the ddpX gene.
  
  

Revision as of 12:10, 10 October 2016


inclusion body induced cell lysis system

Long Description: This part consists of theCpxR responsive promoter (Part:BBa_K135000), a RBS (Part:BBa_B0034), ddpX gene (Part: BBa_K2110004), two terminators (Part:BBa_B0010, Part:BBa_B0012). The CpxR responsive promoter can response to the inclusion body and misfold protein in the periplasm. When this system coexpress with an exogenous gene like the PETase gene, the forming inclusion body will induce the expression of downstream ddpX gene and cause the cell lysis, which provides much convenience to the purification steps followed.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Reference

Previous part <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K339007"_blank">BBa_K339007 </a>acted as our reference, we improved its application by changing the mRFP gene to the ddpX gene.