Difference between revisions of "Part:BBa K2148003"
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===Usage and Biology=== | ===Usage and Biology=== | ||
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===Characterisation=== | ===Characterisation=== | ||
Latest revision as of 23:28, 9 October 2016
atpA promoter/5UTR
A level 0 Phytobrick coded as promoter/5UTR which carries the atpA promoter for expression of genes in Chlamydomonas reinhardtii.
Usage and Biology
This part can be used along with other Level 0 part to create a composite part which is a transcriptional unit with the desired Coding DNA Sequence (CDS).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 543
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Characterisation
References
Drapier D, Suzuki H, Levy H, et al. The Chloroplast atpA Gene Cluster in Chlamydomonas reinhardtii : Functional Analysis of a Polycistronic Transcription Unit. Plant Physiology. 1998;117(2):629-641.
Wannathong T, Waterhouse JC, Young REB, Economou CK, Purton S. New tools for chloroplast genetic engineering allow the synthesis of human growth hormone in the green alga Chlamydomonas reinhardtii. Applied Microbiology and Biotechnology. 2016;100:5467-5477. doi:10.1007/s00253-016-7354-6.