Difference between revisions of "Part:BBa K2148001"
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The aadA gene confers antibiotic resistance to Spectinomycin and Streptomycin. | The aadA gene confers antibiotic resistance to Spectinomycin and Streptomycin. | ||
| − | This part can be used to provide a selection pressure on transformations. | + | This part can be used with other level 0 Phytobrick to create a transcriptional unit to provide a selection pressure on transformations. |
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Revision as of 23:14, 9 October 2016
aadA
A commonly used antibiotic for Chlamydomonas chloroplasts. Confers resistance to spectinomycin and streptomycin
Usage and Biology
The aadA gene confers antibiotic resistance to Spectinomycin and Streptomycin.
This part can be used with other level 0 Phytobrick to create a transcriptional unit to provide a selection pressure on transformations.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 652
- 1000COMPATIBLE WITH RFC[1000]
Characterization
References
Goldschmidt-Clermont M. Transgenic expression of aminoglycoside adenine transferase in the chloroplast: a selectable marker of site-directed transformation of chlamydomonas. Nucleic Acids Research. 1991;19(15):4083-4089.