Difference between revisions of "Part:BBa K2148000"
(→Characterisation) |
|||
| Line 22: | Line 22: | ||
===Characterisation=== | ===Characterisation=== | ||
| + | PCR | ||
| + | ssfasdkfnasdjfasdf.sad | ||
| + | sdaf.das | ||
| + | |||
| + | 1 | ||
| + | 2 | ||
| + | 3 | ||
===References=== | ===References=== | ||
Revision as of 22:44, 9 October 2016
psaA1 promoter + 5
Promoter and 5'UTR sequence for the psaA gene in Chlamydomonas reinhardtii chloroplast, flanked with the Phytobrick fusion sites for a promoter with 5'UTR part. Submitted in the universal acceptor vector (psB1C3), and can be extracted using Bsa1 to give the necessary Phytobrick fusion site overhangs for Golden Gate assembly to produce a transcriptional unit with other Phytobricks.
Usage and Biology
This part can be used along with other Level 0 part to create a composite part which is a transcriptional unit with the desired Coding DNA Sequence (CDS).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Characterisation
PCR
ssfasdkfnasdjfasdf.sad sdaf.das
1 2 3