Difference between revisions of "Part:BBa K1968001:Design"

 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
Nucleotide X at position X was mutated to a X in order to remove a BsaI site, which would have interfered with later assemblies.  
+
Nucleotide G at position 109 was mutated to a A in order to remove a BbsI site, which would have interfered with later MoClo assemblies.  
  
  

Revision as of 19:01, 9 October 2016


Synechocystis Pcpc560 Phytobrick promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2


Design Notes

Nucleotide G at position 109 was mutated to a A in order to remove a BbsI site, which would have interfered with later MoClo assemblies.


Source

The part was ordered as gblock through IDT and was subsequently cloned into PhytoBricks Universal Acceptor plasmids (BBa_P10500).

References