Difference between revisions of "Part:BBa K1957004"
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| − | [[File:T--NRP-UEA-Norwich-- | + | [[File:T--NRP-UEA-Norwich--Sequencing_Data_6072_.jpg|thumb|none|Figure 2: Sequencing data aligned with HydA gene insert using the software Benchling. (A) Screenshot of sequencing data at 28bp (B) Screenshot of sequencing data at 528bp]] |
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Revision as of 14:11, 9 October 2016
HydA subunit of FeFe Hydrogenase
One of the three subunits that make up FeFe Hydrogenase in Shewanella oneidensis. Specifically this is the HydA subunit which is the membrane bound catalytic subunit. This unit is one out of the three subunits that can be used to make a the entire FeFe hydrogenase construct.
DNA of the colony on lane 6 and 7 were sent off for sequencing. After sequencing confirmation it was used for the Golden Gate cloning and submitted into the iGEM registry.
Restriction site incompatibility, [1000], is with regards to MoClo i.e. Golden Gate cloning. As we used this in downstream cloning experiments the restriction site could not be avoided.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1
Illegal BsaI.rc site found at 1261
Illegal SapI site found at 1219
