Difference between revisions of "Part:BBa K2132005:Design"

(→‎Design Notes)
(→‎Source)
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===Source===
 
===Source===
  
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The sequence of hydA hydrogenase gene is from the chromosome of Clostridium acetobutylicum ATCC 824.
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The sequence of hydA hydrogenase gene is from the chromosome of '' Clostridium acetobutylicum '' ATCC 824.
  
 
===References===
 
===References===
 
Paul W. King, Matthew C. Posewitz, Maria L. Ghirardi, Michael Seibert. Functional studies of [FeFe] hydrogenase maturation in an Escherichia coli biosynthetic system.[J]. Journal of Bacteriology, 2006, 188(6):2163-72.
 
Paul W. King, Matthew C. Posewitz, Maria L. Ghirardi, Michael Seibert. Functional studies of [FeFe] hydrogenase maturation in an Escherichia coli biosynthetic system.[J]. Journal of Bacteriology, 2006, 188(6):2163-72.

Revision as of 13:01, 9 October 2016


HydA with SpyTag, Histag and TEV site


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 790
    Illegal AgeI site found at 1300
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1005


Design Notes

We used Histag for protein purification and added the TEV site so that it will be easy to cut off the attached Histag.

Source

The sequence of hydA hydrogenase gene is from the chromosome of Clostridium acetobutylicum ATCC 824.

References

Paul W. King, Matthew C. Posewitz, Maria L. Ghirardi, Michael Seibert. Functional studies of [FeFe] hydrogenase maturation in an Escherichia coli biosynthetic system.[J]. Journal of Bacteriology, 2006, 188(6):2163-72.