Difference between revisions of "Part:BBa K1968000"

Latest revision as of 12:00, 9 October 2016

PA1lac0-1 Phytobrick

Derived from the PA1 promoter of bacteriophage T7, PA1lac0-1 is a LacI-dependent promoter and Isopropyl β-D-1-thiogalactopyranoside (IPTG)-inducible promoter that has been shown to bring about an 8-fold induction in both E. coli and Synechocystis (1).

(1) Guerrero, F., Carbonell, V., Cossu, M., Correddu, D., & Jones, P. R. (2012). Ethylene synthesis and regulated expression of recombinant protein in Synechocystis sp. PCC 6803. PLoS One, 7(11), e50470. doi: 10.1371/journal.pone.0050470

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K1968000 short</partinfo>
-Derived from the PA1 promoter of bacteriophage T7, PA1lac0-1 is a LacI-dependent promoter and Isopropyl &#946;-D-1-thiogalactopyranoside (IPTG)-inducible promoter that has been  shown to bring about an 8-fold induction in both E. coli and Synechocystis (Guerrero et al., 2012).
+Derived from the PA1 promoter of bacteriophage T7, PA1lac0-1 is a LacI-dependent promoter and Isopropyl &#946;-D-1-thiogalactopyranoside (IPTG)-inducible promoter that has been  shown to bring about an 8-fold induction in both E. coli and Synechocystis (1).
-Guerrero, F., Carbonell, V., Cossu, M., Correddu, D., & Jones, P. R. (2012). Ethylene synthesis and regulated expression of recombinant protein in Synechocystis sp. PCC 6803. PLoS One, 7(11), e50470. doi: 10.1371/journal.pone.0050470
+(1) Guerrero, F., Carbonell, V., Cossu, M., Correddu, D., & Jones, P. R. (2012). Ethylene synthesis and regulated expression of recombinant protein in Synechocystis sp. PCC 6803. PLoS One, 7(11), e50470. doi: 10.1371/journal.pone.0050470
 <!-- Add more about the biology of this part here