Difference between revisions of "Part:BBa K2151011"

Latest revision as of 22:23, 8 October 2016

p32-B0034-K592009: Blue chromoprotein under control of strong constitutive promoter

This part consists of amilCP, a blue chromoprotein with a strong RBS, under the control of p32, a strong constitutive bacterial promoter. Its intended use is to serve as a visual promoter-reporter construct in lactic acid bacteria, potentially as an alternative to mCherry which may take longer to become visible. p32 is used as a promoter in expression vectors functioning within Streptococcus thermophilus

We used p32 as a candidate promoter to drive gene expression in both Streptococcus thermophilus and E. coli. Quantification of p32 using GFP revealed promoter strength on par with strong Anderson promoters (81% as strong as J23100, 115% as strong as J23101).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K2151011 short</partinfo>
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+<p>This part consists of amilCP, a blue chromoprotein with a strong RBS, under the control of p32, a strong constitutive bacterial promoter. Its intended use is to serve as a visual promoter-reporter construct in lactic acid bacteria, potentially as an alternative to mCherry which may take longer to become visible. p32 is used as a promoter in expression vectors functioning within <i>Streptococcus thermophilus</i> </p>
+ <p>We used p32 as a candidate promoter to drive gene expression in both <i>Streptococcus thermophilus</i> and <i>E. coli</i>. Quantification of p32 using GFP revealed promoter strength on par with strong Anderson promoters (81% as strong as J23100, 115% as strong as J23101).</p>
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