Difference between revisions of "Part:BBa K2151009"
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<partinfo>BBa_K2151009 short</partinfo> | <partinfo>BBa_K2151009 short</partinfo> | ||
| − | . | + | <p>This part consists of amilCP, a blue chromoprotein, with a strong ribosome binding site under the control of pHLBA, a strong constitutive bacterial promoter. Its intended use is to serve as a visual promoter-reporter construct in lactic acid bacteria, acting as an alternative to mCherry which can take longer to become visible. pHLBA was initially identified in studies of <i>Lactobacillus bulgaricus</i> where it drives the expression of histone-like proteins. pHLBA is likely functional in all lactic acid bacteria. </p> |
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| + | <p>We used pHLBA as a candidate promoter to drive gene expression in both <i>Streptococcus thermophilus</i> and <i>E. coli</i>. Quantification of pHLBA using GFP in <i>E. coli</i> revealed promoter strength on par with strong Anderson promoters (93% as strong as J23100, 132% as strong as J23101).</p> | ||
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Latest revision as of 22:14, 8 October 2016
pHLBA-B0034-K592009: Blue chromoprotein under control of strong constitutive promoter
This part consists of amilCP, a blue chromoprotein, with a strong ribosome binding site under the control of pHLBA, a strong constitutive bacterial promoter. Its intended use is to serve as a visual promoter-reporter construct in lactic acid bacteria, acting as an alternative to mCherry which can take longer to become visible. pHLBA was initially identified in studies of Lactobacillus bulgaricus where it drives the expression of histone-like proteins. pHLBA is likely functional in all lactic acid bacteria.
We used pHLBA as a candidate promoter to drive gene expression in both Streptococcus thermophilus and E. coli. Quantification of pHLBA using GFP in E. coli revealed promoter strength on par with strong Anderson promoters (93% as strong as J23100, 132% as strong as J23101).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]