Difference between revisions of "Part:BBa K2151005"

Revision as of 21:37, 8 October 2016

p32-E5501: GFP under control of strong constitutive promoter

This part consists of E5501 (GFP with medium ribosome binding site) under the control of p32, a strong constitutive bacterial promoter. Its intended use is to serve as a promoter-reporter construct in lactic acid bacteria. p32 is used as a promoter in expression vectors functioning within Streptococcus thermophilus

We used p32 as a candidate promoter to drive gene expression in both Streptococcus thermophilus and E. coli. Quantification of p32 using GFP revealed promoter strength on par with strong Anderson promoters (81% as strong as J23100, 115% as strong as J23101).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 721

@@ Line 5: / Line 5: @@
 <p>This part consists of E5501 (GFP with medium ribosome binding site) under the control of p32, a strong constitutive bacterial promoter. Its intended use is to serve as a promoter-reporter construct in lactic acid bacteria. p32 is used as a promoter in expression vectors functioning within <i>Streptococcus thermophilus</i> </p>
-  <p>We used p32 as a candidate promoter to drive gene expression in both <i>Streptococcus thermophilus</i> and <i>E. coli</i>. Quantification of p32 using GFP revealed promoter strength on par with strong Anderson promoters (81% as strong as J23100, 115% as strong as J23101).</p></p>
+  <p>We used p32 as a candidate promoter to drive gene expression in both <i>Streptococcus thermophilus</i> and <i>E. coli</i>. Quantification of p32 using GFP revealed promoter strength on par with strong Anderson promoters (81% as strong as J23100, 115% as strong as J23101).</p>
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