Difference between revisions of "Part:BBa K2151000:Design"

(→‎Design Notes)
(Added source)
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===Design Notes===
 
===Design Notes===
  
−
The sequence was not altered for use in <i>E. coli</i> as the aim was to achieve expression in <i>S. thermophilus</i>
+
The sequence was not altered for use in <i>E. coli</i> as the aim was to achieve expression in <i>S. thermophilus</i>.
  
 
===Source===
 
===Source===
  
 
<ul>
 
<ul>
−
<li>Sequence obtained from 'Highly efficient production of the staphylococcal nuclease reporter in Lactobacillus bulgaricus governed by the promoter of the hlbA gene'.</li>
+
<li>Sequence obtained from 'ISOLATION AND CHARACTERIZATION OF CHROMOSOMAL PROMOTERS OF STREPTOCOCCUS-SALIVARIUS SUBSP THERMOPHILUS'.</li>
  
 
<li>Synthesized by IDT as an oligo-nucleotide</li>
 
<li>Synthesized by IDT as an oligo-nucleotide</li>
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===References===
 
===References===
  
−
  <p>Chouayekh, H., Serror, P., Boudebbouze, S. and Maguin, E. (2009) 'Highly efficient production of the staphylococcal nuclease reporter in Lactobacillus bulgaricus governed by the promoter of the hlbA gene', Fems Microbiology Letters, 293(2), pp. 232-239.</p>
+
  <p>Slos, P., Bourquin, J. C., Lemoine, Y. and Mercenier, A. (1991) 'ISOLATION AND CHARACTERIZATION OF CHROMOSOMAL PROMOTERS OF STREPTOCOCCUS-SALIVARIUS SUBSP THERMOPHILUS', Applied and Environmental Microbiology, 57(5), pp. 1333-1339.</p>

Revision as of 20:53, 8 October 2016


pHLBA, strong constitutive S. thermophilus promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was not altered for use in E. coli as the aim was to achieve expression in S. thermophilus.

Source

  • Sequence obtained from 'ISOLATION AND CHARACTERIZATION OF CHROMOSOMAL PROMOTERS OF STREPTOCOCCUS-SALIVARIUS SUBSP THERMOPHILUS'.
  • Synthesized by IDT as an oligo-nucleotide

References

Slos, P., Bourquin, J. C., Lemoine, Y. and Mercenier, A. (1991) 'ISOLATION AND CHARACTERIZATION OF CHROMOSOMAL PROMOTERS OF STREPTOCOCCUS-SALIVARIUS SUBSP THERMOPHILUS', Applied and Environmental Microbiology, 57(5), pp. 1333-1339.