Difference between revisions of "Part:BBa K2151000"
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| − | <p>This part consists of pHLBA, a strong constitutive bacterial promoter, contained within pSB1C3. pHLBA was initially identified in studies of <i>Lactobacillus bulgaricus</i> where it drives the expression of histone-like proteins. pHLBA is likely functional in all lactic acid bacteria. </p> | + | <p>This part consists of pHLBA, a strong constitutive bacterial promoter, contained within pSB1C3. Its intended use is to promote transcription of an inserted gene. pHLBA was initially identified in studies of <i>Lactobacillus bulgaricus</i> where it drives the expression of histone-like proteins. pHLBA is likely functional in all lactic acid bacteria. </p> |
<p>We used pHLBA as a candidate promoter to drive gene expression in both <i>Streptococcus thermophilus</i> and <i>E. coli</i>. Quantification of pHLBA using GFP revealed promoter strength on par with strong Anderson promoters (93% as strong as J23100, 132% as strong as J23101).</p> | <p>We used pHLBA as a candidate promoter to drive gene expression in both <i>Streptococcus thermophilus</i> and <i>E. coli</i>. Quantification of pHLBA using GFP revealed promoter strength on par with strong Anderson promoters (93% as strong as J23100, 132% as strong as J23101).</p> | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
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Revision as of 20:42, 8 October 2016
pHLBA, strong constitutive S. thermophilus promoter
This part consists of pHLBA, a strong constitutive bacterial promoter, contained within pSB1C3. Its intended use is to promote transcription of an inserted gene. pHLBA was initially identified in studies of Lactobacillus bulgaricus where it drives the expression of histone-like proteins. pHLBA is likely functional in all lactic acid bacteria.
We used pHLBA as a candidate promoter to drive gene expression in both Streptococcus thermophilus and E. coli. Quantification of pHLBA using GFP revealed promoter strength on par with strong Anderson promoters (93% as strong as J23100, 132% as strong as J23101).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]