Difference between revisions of "Part:BBa K2114000"
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===Usage and Biology===
 
===Usage and Biology===
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The PCotYZ promoter from Bacillus subtilis plays a role in the sporulation of B. subtilis. The Promoter is located in the cotVWXYZ gene cluster and drives the expression of the late-stage spore crust proteins CotY and CotZ[1]
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The PCotYZ promoter from Bacillus subtilis plays an important role in the sporulation of B. subtilis. The Promoter is located in the cotVWXYZ gene cluster and drives the expression of the late-stage spore crust proteins CotY and CotZ [1]. We included the naturally occurring ribosome binding site for cotZ to the promoter while maintaining the BioBrick compatibility. The improved promoter can be used for the 3A assembly with a coding region and cloned into an integration vector. The resulting device can be transformed into B. subtilis for integration and expression.
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Revision as of 20:41, 8 October 2016


PCotYZ-RBS

PCotYZ promoter from Bacillus subtilis with ribosome binding site.

Usage and Biology

The PCotYZ promoter from Bacillus subtilis plays an important role in the sporulation of B. subtilis. The Promoter is located in the cotVWXYZ gene cluster and drives the expression of the late-stage spore crust proteins CotY and CotZ [1]. We included the naturally occurring ribosome binding site for cotZ to the promoter while maintaining the BioBrick compatibility. The improved promoter can be used for the 3A assembly with a coding region and cloned into an integration vector. The resulting device can be transformed into B. subtilis for integration and expression.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]