Difference between revisions of "Part:BBa K2020001"
 
Line 3: Line 3:
 
<partinfo>BBa_K2020001 short</partinfo>
 
<partinfo>BBa_K2020001 short</partinfo>
  
−
This DNA sequence can be used to express subtilisin E in ''Escherichia coli'' and simultaneously secret the enzyme into the periplasm of the cell. Subtilisin E is an alkaline serine protease which non-specifically digests proteins. <br>
+
This DNA sequence can be used to express subtilisin E in ''Escherichia coli'' and simultaneously secret the enzyme into the periplasm of the cell. <br>
 
This composite part consists of the leader sequence pelB ([[Part:BBa_J32015|BBa_J32015]]) and the subtilisin E gene ([[Part:BBa_K2020000|BBa_K2020000]]).
 
This composite part consists of the leader sequence pelB ([[Part:BBa_J32015|BBa_J32015]]) and the subtilisin E gene ([[Part:BBa_K2020000|BBa_K2020000]]).
  
−
<!--
+
 
 
===Usage and Biology===
 
===Usage and Biology===
−
-->
+
 
 +
Subtilisin E is an alkaline serine protease which non-specifically digests proteins. It is naturally produced by ''Bacillus subtilis''.
 +
 
  
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Latest revision as of 20:17, 8 October 2016


subtilisin E gene with leader sequence pelB

This DNA sequence can be used to express subtilisin E in Escherichia coli and simultaneously secret the enzyme into the periplasm of the cell.
This composite part consists of the leader sequence pelB (BBa_J32015) and the subtilisin E gene (BBa_K2020000).


Usage and Biology

Subtilisin E is an alkaline serine protease which non-specifically digests proteins. It is naturally produced by Bacillus subtilis.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 54
  • 1000
    COMPATIBLE WITH RFC[1000]