Difference between revisions of "Part:BBa K2151000"
(Added long description using main body.) |
|||
| Line 3: | Line 3: | ||
<partinfo>BBa_K2151000 short</partinfo> | <partinfo>BBa_K2151000 short</partinfo> | ||
| − | + | ||
| + | <p>This part consists of pHLBA, a strong constitutive bacterial promoter, contained within pSB1C3. pHLBA was initially identified in studies of <i>Lactobacillus bulgaricus</i> where it drives the expression of histone-like proteins. pHLBA is likely functional in all lactic acid bacteria. </p> | ||
| + | |||
| + | <p>We used pHLBA as a candidate promoter to drive gene expression in both <i>Streptococcus thermophilus</i> and <i>E. coli</i>. Quantification of pHLBA using GFP revealed promoter strength on par with strong Anderson promoters (93% as strong as J23100, 132% as strong as J23101).</p> | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 18:54, 8 October 2016
pHLBA, strong constitutive S. thermophilus promoter
This part consists of pHLBA, a strong constitutive bacterial promoter, contained within pSB1C3. pHLBA was initially identified in studies of Lactobacillus bulgaricus where it drives the expression of histone-like proteins. pHLBA is likely functional in all lactic acid bacteria.
We used pHLBA as a candidate promoter to drive gene expression in both Streptococcus thermophilus and E. coli. Quantification of pHLBA using GFP revealed promoter strength on par with strong Anderson promoters (93% as strong as J23100, 132% as strong as J23101).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]