Difference between revisions of "Part:BBa K2151000:Design"
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===Design Notes=== | ===Design Notes=== | ||
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| + | The sequence was not altered for use in <i>E. coli</i> as the aim was to achieve expression in <i>S. thermophilus</i> | ||
===Source=== | ===Source=== | ||
Revision as of 18:19, 8 October 2016
pHLBA, strong constitutive S. thermophilus promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence was not altered for use in E. coli as the aim was to achieve expression in S. thermophilus
Source
- Sequence obtained from 'Highly efficient production of the staphylococcal nuclease reporter in Lactobacillus bulgaricus governed by the promoter of the hlbA gene'.
- Synthesized by IDT as an oligo-nucleotide
References
Chouayekh, H., Serror, P., Boudebbouze, S. and Maguin, E. (2009) 'Highly efficient production of the staphylococcal nuclease reporter in Lactobacillus bulgaricus governed by the promoter of the hlbA gene', Fems Microbiology Letters, 293(2), pp. 232-239.