Difference between revisions of "Part:BBa K1969003"
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<partinfo>BBa_K1969003 short</partinfo> | <partinfo>BBa_K1969003 short</partinfo> | ||
− | This is the full length of yeast ADH1 promoter cloned from genomic DNA of Saccharomyces cerevisiae. | + | This is the full length of yeast ADH1 promoter cloned from genomic DNA of Saccharomyces cerevisiae. Although originally thought to be expressed constitutively, ADH1 promoter mediated ADH1 transcription is repressed when cells are grown on a non-fermentable carbon source such as ethanol or glycerol. Thus when culturing yeast with non-fermentable medium, researchers should pay attention to the expression level. |
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Latest revision as of 14:22, 8 October 2016
Full length S. cerevisiae ADH1 promoter
This is the full length of yeast ADH1 promoter cloned from genomic DNA of Saccharomyces cerevisiae. Although originally thought to be expressed constitutively, ADH1 promoter mediated ADH1 transcription is repressed when cells are grown on a non-fermentable carbon source such as ethanol or glycerol. Thus when culturing yeast with non-fermentable medium, researchers should pay attention to the expression level.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 90