Difference between revisions of "Part:BBa K2010005"

 
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<partinfo>BBa_K2010005 short</partinfo>
 
<partinfo>BBa_K2010005 short</partinfo>
  
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This His-tag is a sequence that allows a conjunct protein to be purified. The tag is composed of repetitions of histidines. This is an alternate version of BBa_K2010004, but serves the same function.  
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PETase is the PET(poly(ethylene terephtalate))-degrading enzyme first identified in Ideonella sakaiensis. This sequence is the E. coli K12 optimized DNA sequence for PETase.
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This part includes a T7 promoter (BBa_I712074), RBS 34 (BBa_B0034), and PETase fused with a His-tag, for purification. In addition, this part includes sfGFP, a variant of GFP that facilitates protein folding, and ompT, a secretion tag to secrete PETase to the extracellular medium.  
  
 
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Revision as of 07:18, 8 October 2016


ompT + sfGFP + PETase (PET-degrading enzyme, origin I. sakaiensis)

PETase is the PET(poly(ethylene terephtalate))-degrading enzyme first identified in Ideonella sakaiensis. This sequence is the E. coli K12 optimized DNA sequence for PETase.

This part includes a T7 promoter (BBa_I712074), RBS 34 (BBa_B0034), and PETase fused with a His-tag, for purification. In addition, this part includes sfGFP, a variant of GFP that facilitates protein folding, and ompT, a secretion tag to secrete PETase to the extracellular medium.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 809
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1227