Difference between revisions of "Part:BBa K1963004"

 
Line 3: Line 3:
 
<partinfo>BBa_K1963004 short</partinfo>
 
<partinfo>BBa_K1963004 short</partinfo>
  
This is a multi-sequence system for small RNA (sRNA) production in an E. coli host - or especially with co-expression of E. coli Hfq. The system is based on the BBa_K1963002 template and has the proD strong promoter, followed by a short antisense sequence targeting the 5' end of the fliC transcript, followed by the E. coli micC hairpin, followed by the strong terminator sequence T1/TE. The anti fliC sRNA is placed betweeen C-193 and T-194. The system should impair motility of E. coli.
+
This is a multi-sequence system for small RNA (sRNA) production in an <i>E. coli</i> host - or especially with co-expression of <i>E. coli</i> Hfq <partinfo>BBa_K1963000</i>. The system is based on the <partinfo>BBa_K1963002</partinfo> template and has the <i>proD</i> strong promoter, followed by a short antisense sequence targeting the 5' end of the <i>fliC</i> transcript, followed by the <i>E. coli micC</i> hairpin, followed by the strong terminator sequence T1/TE. The anti <i>fliC</i> sRNA is placed betweeen C-193 and T-194. The system should impair motility of <i>E. coli</i>.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 20:46, 7 October 2016


An sRNA system for down-regulation of E. coli fliC encoding flagellin.

This is a multi-sequence system for small RNA (sRNA) production in an E. coli host - or especially with co-expression of E. coli Hfq No part name specified with partinfo tag. template and has the proD strong promoter, followed by a short antisense sequence targeting the 5' end of the fliC transcript, followed by the E. coli micC hairpin, followed by the strong terminator sequence T1/TE. The anti fliC sRNA is placed betweeen C-193 and T-194. The system should impair motility of E. coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 101