Difference between revisions of "Part:BBa K2060002"

 
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<partinfo>BBa_K2060002 short</partinfo>
 
<partinfo>BBa_K2060002 short</partinfo>
  
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The pBAD::LUXoperon (BBa_K325909) has been well characterised and the bacteria containing this biobrick is able to produce blue light in vivo after induction by Arabinose.  
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The pBAD::LUXoperon (<a href="https://parts.igem.org/Part:BBa_K325909">BBa_K325909</a>) has been well characterised and the bacteria containing this biobrick is able to produce blue light in vivo after induction by Arabinose.  
  
 
We have generated a part that has RBS-mKeima at the 3' end of the LUXoperon in order to attempt to bring about a stroke shift in light output from blue>red.  
 
We have generated a part that has RBS-mKeima at the 3' end of the LUXoperon in order to attempt to bring about a stroke shift in light output from blue>red.  

Revision as of 11:35, 7 October 2016


LUXoperon with mKeima

The pBAD::LUXoperon (<a href="https://parts.igem.org/Part:BBa_K325909">BBa_K325909</a>) has been well characterised and the bacteria containing this biobrick is able to produce blue light in vivo after induction by Arabinose.

We have generated a part that has RBS-mKeima at the 3' end of the LUXoperon in order to attempt to bring about a stroke shift in light output from blue>red.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
    Illegal NheI site found at 4171
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3169
    Illegal BamHI site found at 1144
    Illegal XhoI site found at 3999
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 5558
    Illegal BsaI.rc site found at 2567
    Illegal SapI site found at 961
    Illegal SapI.rc site found at 5883