Difference between revisions of "Part:BBa K2052014:Design"

 
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===References===
 
===References===
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Krogfelt KA., Bergmans H., Klemm P. (1990, June) “Direct evidence that the FimH protein is the mannose-specific adhesin of Escherichia coli type 1 fimbriae”
 +
 +
Sauer MM, Jakob RP, Eras J, Baday S, Eriş D, Navarra G, Bernèche S, Ernst B, Maier T,
 +
Glockshuber R. (2016 March 7). “Catch-bond mechanism of the bacterial adhesin FimH”
 +
 +
Kelly, K. A., Jones, D. A., (2003). “Isolation of a Colon Tumor Specific Binding Peptide Using
 +
Phage Display Selection”
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Figure 1. iGEM 2015 Harvard BioDesign . Retrieved from https://parts.igem.org/Part:BBa_K1850010
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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1550331/
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Induction protocol http://www.embl.de/pepcore/pepcore_services/protein_expression/ecoli/

Revision as of 16:46, 6 October 2016


FimH site directed mutated with RPMrel


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1173
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1113
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

....Enter any design considerations you had to deal with during the detailed design of the sequence.


Source

....Enter the source of this part. For example, does it come from some genomic sequence?

References

Krogfelt KA., Bergmans H., Klemm P. (1990, June) “Direct evidence that the FimH protein is the mannose-specific adhesin of Escherichia coli type 1 fimbriae”

Sauer MM, Jakob RP, Eras J, Baday S, Eriş D, Navarra G, Bernèche S, Ernst B, Maier T, Glockshuber R. (2016 March 7). “Catch-bond mechanism of the bacterial adhesin FimH”

Kelly, K. A., Jones, D. A., (2003). “Isolation of a Colon Tumor Specific Binding Peptide Using Phage Display Selection”

Figure 1. iGEM 2015 Harvard BioDesign . Retrieved from https://parts.igem.org/Part:BBa_K1850010

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1550331/


Induction protocol http://www.embl.de/pepcore/pepcore_services/protein_expression/ecoli/