Difference between revisions of "Part:BBa K1959002"
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<partinfo>BBa_K1959002 short</partinfo> | <partinfo>BBa_K1959002 short</partinfo> | ||
− | This part codes for beta-carotene hydroxylase, which is needed for astaxanthin biosynthesis. | + | This part codes for beta-carotene hydroxylase(BHY), which is needed for astaxanthin biosynthesis. The BHY cds is fused with Pea transpsit peptide to help BHY folding correctly in Orazy ssativa cell. BHY catalyzes the convertion of beta-carotene to zeaxanthin. The part is original from Haematococcus pluvialis and codon optimization has been made in order to efficiently |
express in Orazy sativa. | express in Orazy sativa. | ||
Revision as of 03:36, 6 October 2016
Modification of β-carotene hydroxylase (BHY)
This part codes for beta-carotene hydroxylase(BHY), which is needed for astaxanthin biosynthesis. The BHY cds is fused with Pea transpsit peptide to help BHY folding correctly in Orazy ssativa cell. BHY catalyzes the convertion of beta-carotene to zeaxanthin. The part is original from Haematococcus pluvialis and codon optimization has been made in order to efficiently express in Orazy sativa.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 334
Illegal NgoMIV site found at 726
Illegal NgoMIV site found at 793 - 1000COMPATIBLE WITH RFC[1000]