Difference between revisions of "Part:BBa K2020002:Design"

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(→‎Source)
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The sequences of the BioBrick parts were obtained through the Registry.  
 
The sequences of the BioBrick parts were obtained through the Registry.  
−
* LacI promoter [[Part:BBa_R0010|BBa_R0010]]
+
{| class="wikitable" style="text-align:center"
−
* RBS [[Part:BBa_B0034|BBa_B0034]]
+
|-
−
* leader sequence pelB ([[Part:BBa_J32015|BBa_J32015]]) + subtilisin E gene ([[Part:BBa_K2020000|BBa_K2020000]])
+
! part !! colspan="2"| BioBrick No.
−
* terminator [[Part:BBa_B0010|BBa_B0010]]
+
|-
 +
| LacI promoter || colspan="2"| [[Part:BBa_R0010|BBa_R0010]]
 +
|-
 +
| RBS || colspan="2"| [[Part:BBa_B0034|BBa_B0034]]
 +
|-
 +
| rowspan="2" | CDS || colspan="2"| [[Part:BBa_K2020001|BBa_K2020001]]
 +
|-
 +
| [[Part:BBa_J32015|BBa_J32015]] || [[Part:BBa_K2020000|BBa_K2020000]]
 +
|-
 +
| terminator || colspan="2"| [[Part:BBa_B0010|BBa_B0010]]
 +
|-
 +
|}
 
The construct was ordered at IDT.
 
The construct was ordered at IDT.
  
 
===References===
 
===References===

Revision as of 17:00, 5 October 2016


expression system for subtilisin E in E. coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 280
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence of the subtilisin E gene from Bacillus subtilis was codon optimized for E. coli with the DNA and protein sequence analysis software "Geneious" and additionally with the "Codon Optimization Tool" from IDT.


Source

The sequences of the BioBrick parts were obtained through the Registry.

part BioBrick No.
LacI promoter BBa_R0010
RBS BBa_B0034
CDS BBa_K2020001
BBa_J32015 BBa_K2020000
terminator BBa_B0010

The construct was ordered at IDT.

References