Difference between revisions of "Part:BBa K2020002:Design"
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The sequences of the BioBrick parts were obtained through the Registry. | The sequences of the BioBrick parts were obtained through the Registry. | ||
| − | * LacI promoter [[Part:BBa_R0010 | BBa_R0010]] | + | * LacI promoter [[Part:BBa_R0010|BBa_R0010]] |
| − | * RBS [[Part:BBa_B0034 | BBa_B0034]] | + | * RBS [[Part:BBa_B0034|BBa_B0034]] |
| − | * leader sequence pelB ([[Part:BBa_J32015|BBa_J32015]]) + subtilisin E gene ([[Part:BBa_K2020000 | BBa_K2020000]]) | + | * leader sequence pelB ([[Part:BBa_J32015|BBa_J32015]]) + subtilisin E gene ([[Part:BBa_K2020000|BBa_K2020000]]) |
| − | * terminator [[Part:BBa_B0010 | BBa_B0010]] | + | * terminator [[Part:BBa_B0010|BBa_B0010]] |
The construct was ordered at IDT. | The construct was ordered at IDT. | ||
===References=== | ===References=== | ||
Revision as of 16:49, 5 October 2016
expression system for subtilisin E in E. coli
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 280
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence of the subtilisin E gene from Bacillus subtilis was codon optimized for E. coli with the DNA and protein sequence analysis software "Geneious" and additionally with the "Codon Optimization Tool" from IDT.
Source
The sequences of the BioBrick parts were obtained through the Registry.
- LacI promoter BBa_R0010
- RBS BBa_B0034
- leader sequence pelB (BBa_J32015) + subtilisin E gene (BBa_K2020000)
- terminator BBa_B0010
The construct was ordered at IDT.