Difference between revisions of "Part:BBa K2017003"

Revision as of 14:23, 3 October 2016

SAGTI linker + Luciferase

Firefly luciferase protein. It oxidates its substrate luciferin, emitting light during the process. It can act as a reporter protein when used with a promoter. This part is made to be fused downstream to other coding sequence, so it includes the SAGTI linker in order to let the luciferase acquire the correct tertiary structure.

The luciferase does not include ATG (Met) to initiate translation, as it needs to be fused to other coding sequence on the 5'. The translation must begin in the coding sequence fused upstream. The linker includes a random nucleotide in 5', to change the reading frame of the luciferase. That way, it will not be translated unless an indel is produced in the coding region to which the part is fused.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 822

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2017003 short</partinfo>
-Firefly luciferase protein. It oxidates its substrate luciferin, emitting light during the process. It can act as a reporter protein when used with a promoter. This part is made to be fused to the 3' with other coding sequence, so it includes the SAGTI linker in order to let the luciferase acquire the correct tertiary structure.
+Firefly luciferase protein. It oxidates its substrate luciferin, emitting light during the process. It can act as a reporter protein when used with a promoter. This part is made to be fused downstream to other coding sequence, so it includes the SAGTI linker in order to let the luciferase acquire the correct tertiary structure.
-The luciferase does not include ATG (Met) to initiate translation, as it needs to be fused to 3' of other coding sequence. The translation must begin in the coding sequence fused to the 3'.
+The luciferase does not include ATG (Met) to initiate translation, as it needs to be fused to other coding sequence on the 5'. The translation must begin in the coding sequence fused upstream.
 The linker includes a random nucleotide in 5', to change the reading frame of the luciferase. That way, it will not be translated unless an indel is produced in the coding region to which the part is fused.