Difference between revisions of "Part:BBa K2036033"

Revision as of 09:08, 3 October 2016

PP2CA-TTADH1-pCyc-Kozak-SnRK2.2-TTADH1-pCyc

Part of genetic circuit in the eukaryotic version of signal filter ([http://2016.igem.org/Team:HUST-China Details see to HUST-China 2016 wiki]).
Main function:
We construct this circuit to verify the interaction between PP2CA(BBa_K2036005) and SnRK2.2(BBa_K2036003) especially the inhibiting effect of PP2CA to SnRK2.2

Charactor rating circuit of PP2CA and SnRK2.2

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 90
Illegal BamHI site found at 661
Illegal BamHI site found at 806
Illegal BamHI site found at 918
Illegal BamHI site found at 1816
Illegal BamHI site found at 2473
Illegal XhoI site found at 65
Illegal XhoI site found at 875
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 48
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2036033 short</partinfo>
-Part of genetic circuit in the eukaryotic version of signal filter.
+Part of genetic circuit in the eukaryotic version of signal filter ([http://2016.igem.org/Team:HUST-China Details see to HUST-China 2016 wiki]).
 <br>
 Main function:<br>
 We construct this circuit to verify the interaction between PP2CA([https://parts.igem.org/Part:BBa_K2036005 BBa_K2036005]) and SnRK2.2([https://parts.igem.org/Part:BBa_K2036003 BBa_K2036003]) especially the inhibiting effect of PP2CA to SnRK2.2<br>
-[[File:T--HUST-China--Description-Fig-prokaryote.png|thumb|500px|center|Charactor rating circuit of PP2CA and SnRK2.2]]
+[[File:T--HUST-China--Experiments-Fig4-2.png|thumb|400px|center|Charactor rating circuit of PP2CA and SnRK2.2]]
 <!-- Add more about the biology of this part here