Difference between revisions of "Part:BBa K2036023"

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<partinfo>BBa_K2036023 short</partinfo>
 
<partinfo>BBa_K2036023 short</partinfo>
  
This part is built by In-Fusion cloning method and together with [https://parts.igem.org/Part:BBa_K2036021 BBa_K2036021] and [https://parts.igem.org/Part:BBa_K2036023 BBa_K2036023] to further assemble [https://parts.igem.org/Part:BBa_K2036024 BBa_K2036024]
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This part is built by In-Fusion cloning method and together with [https://parts.igem.org/Part:BBa_K2036021 BBa_K2036021] and [https://parts.igem.org/Part:BBa_K2036023 BBa_K2036023] to further assemble [https://parts.igem.org/Part:BBa_K2036024 BBa_K2036024]. pRM can be negatively regulated by Cro. ilDH and beta-glactosidase are important enzyme in celluler metobolism.
 
[[File:T--HUST-China--Experiments-Fig15.png|thumb|350px|center|Fig1: Prokaryote application plasmid for lactose intolerance]]
 
[[File:T--HUST-China--Experiments-Fig15.png|thumb|350px|center|Fig1: Prokaryote application plasmid for lactose intolerance]]
  

Revision as of 08:43, 3 October 2016


RBS-iLDH-TT-pRM-RBS-beta-galactosidase

This part is built by In-Fusion cloning method and together with BBa_K2036021 and BBa_K2036023 to further assemble BBa_K2036024. pRM can be negatively regulated by Cro. ilDH and beta-glactosidase are important enzyme in celluler metobolism.

Fig1: Prokaryote application plasmid for lactose intolerance

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 737
    Illegal BamHI site found at 776
    Illegal BamHI site found at 1468
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]