Difference between revisions of "Part:BBa K1033906"
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[[Image:tsp3.png]] | [[Image:tsp3.png]] | ||
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Revision as of 08:43, 3 October 2016
tsPurple, purple chromoprotein
This chromoprotein (also known as TinselPurple) naturally exhibits strong purple color when expressed.
Usage and Biology
This part is useful as a reporter.
iGEM2013 Uppsala: The images above show E coli constitutively expressing the chromoproteins amilCP BBa_K592009 and tsPurple BBa_K1033906 from the high copy plasmid pSB1C3 from the promoters J23116 and J23110.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Structure and SWISS MODEL Homology Modelling Report
(The following information is the contribution of SVCE_Chennai)
The following sections give a detailed information on this chromoprotein done by its in silico analysis.
3D Structure
Cartoon representation of tsPurple.
QMEANnorm = 0.02
Cβ = -0.91
All Atom = 0.52
Solvation = -1.19
Torsion = 0.32
The best match during structure prediction.
| Template | Seq. Identity | Oligo-state | Found by | Method | Resolution | Seq Similarity | Range | Coverage | Description |
|---|---|---|---|---|---|---|---|---|---|
| 4ohs.1.A | 86.61 | homotetramer | HHblits | X-Ray | 2.19Å | 0.58 | 2 - 222 | 0.98 | FAR-RED FLUORESCENT PROTEIN AQ143 |
What about Ligands?
| Ligand | Added to Model | Description |
|---|---|---|
| CL | ✕ - Not biologically relevant. | CHLORIDE ION |
| CL | ✕ - Not biologically relevant. | CHLORIDE ION |
| CL | ✕ - Not biologically relevant. | CHLORIDE ION |
