Difference between revisions of "Part:BBa K2092005:Design"
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===Design Notes=== | ===Design Notes=== | ||
- Codon optimized the sequence with Thermo Fisher Scientific. | - Codon optimized the sequence with Thermo Fisher Scientific. | ||
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- Gene synthesized by IDT. | - Gene synthesized by IDT. | ||
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- Calculated the RBS strength by using the online tool SalisLab RBS Calculator. | - Calculated the RBS strength by using the online tool SalisLab RBS Calculator. | ||
Revision as of 22:43, 2 October 2016
alcR, ethanol-activated transcription factor from A. nidulans (incl. RBS)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2568
Illegal BamHI site found at 1572
Illegal BamHI site found at 2310 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1276
Illegal AgeI site found at 1404
Illegal AgeI site found at 1935 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
- Codon optimized the sequence with Thermo Fisher Scientific. - Gene synthesized by IDT. - Calculated the RBS strength by using the online tool SalisLab RBS Calculator.
Source
Aspergillus nidulans. The protein was first extracted and characterized by <a href="https://www.ncbi.nlm.nih.gov/protein/AAQ06627.">Felenbok et al. (1988)</a> [1]. This version is codon optimized for E coli by Thermo Fisher Scientific.
References
[1] Felenbok,B., Sequeval,D., Mathieu,M., Sibley,S., Gwynne,D.I. & Davies,R.W. (1988). The ethanol regulon in Aspergillus nidulans: characterization and sequence of the positive regulatory gene alcR. Gene, 73(2), 385-396.