Difference between revisions of "Part:BBa K2010000"
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This sequence is the E. coli K12 optimized DNA sequence for PETase. | This sequence is the E. coli K12 optimized DNA sequence for PETase. | ||
| − | This part includes a promoter, RBS, and PETase fused with a His-tag, for purification. | + | This part includes a T7 promoter (BBa_I712074), RBS 34 (BBa_B0034), and PETase fused with a His-tag, for purification. |
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Revision as of 19:18, 2 October 2016
PETase (PET-degrading enzyme, origin I. sakaiensis)
PETase is the PET(poly(ethylene terephtalate))-degrading enzyme first identified in Ideonella sakaiensis. This sequence is the E. coli K12 optimized DNA sequence for PETase.
This part includes a T7 promoter (BBa_I712074), RBS 34 (BBa_B0034), and PETase fused with a His-tag, for purification.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 426