Difference between revisions of "Part:BBa K2020042:Design"
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Since tRNA and Synthetases have own promotors and since they are frequently used in low copy plasmids, you should assemble tRNA and Syntehtases on a plasmid (e.g. pACYC) which fits your needs.
 
Since tRNA and Synthetases have own promotors and since they are frequently used in low copy plasmids, you should assemble tRNA and Syntehtases on a plasmid (e.g. pACYC) which fits your needs.
  
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Team Aachen 2016 ( http://2016.igem.org/Team:Aachen ) used tRNA in pACYC with p15A-ORI, Ipp-Promoter, Terminator, Gent-Resistance. Together with an ncAA-Synthetase (For example: BBa_K2020050) with own promoter and Terminator.
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[http://2016.igem.org/Team:Aachen Team Aachen 2016] used tRNA in pACYC with p15A-ORI, Ipp-Promoter, Terminator, Gent-Resistance. Together with an ncAA-Synthetase (For example: BBa_K2020050) with own promoter and Terminator.
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Revision as of 13:47, 2 October 2016


Mj-tRNA with amber anticodon for incorporating ncAA in E.coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Since tRNA and Synthetases have own promotors and since they are frequently used in low copy plasmids, you should assemble tRNA and Syntehtases on a plasmid (e.g. pACYC) which fits your needs.

[http://2016.igem.org/Team:Aachen Team Aachen 2016] used tRNA in pACYC with p15A-ORI, Ipp-Promoter, Terminator, Gent-Resistance. Together with an ncAA-Synthetase (For example: BBa_K2020050) with own promoter and Terminator.


Source

wild type tyrosyl Methanococcus janaschii tRNA:Synthetase-pair with anticodon changed to Amber Anticodon CTA. Obtained via iGEM Team 2014 Austin, Texas.

References