Difference between revisions of "Part:BBa K2042008"

Revision as of 18:50, 1 October 2016

Engineered D-Lactate dehydrogenase (D-LDH*)

This is the coding sequence of D-lactate deshydrogenase from Lactobacillus delbrueckii 11842. The sequence was condon optimized for Pseudomonas putida and point mutations on three amino acids (D176S, I177R, F178T) were found to efficiently use both NADH and NADPH as cofactors. This enzyme would be useful, as the activity of such an enzyme will not be limited by the availability of NADH or NADPH.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 630
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K2042008 short</partinfo>
-This is the coding sequence of lactate deshydrogenase that can super efficiently use NADPH and NADH as cofactor. Point mutations on three amino acids (D176S, I177R, F178T) resulted in a modified enzyme designated as D-LDH*.
+This is the coding sequence of D-lactate deshydrogenase from Lactobacillus delbrueckii 11842. The sequence was condon optimized for Pseudomonas putida and point mutations on three amino acids (D176S, I177R, F178T) were found to efficiently use both NADH and NADPH as cofactors. This enzyme would be useful, as the activity of such an enzyme will not be limited by the availability of NADH or NADPH.
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