Difference between revisions of "Part:BBa K401001:Experience"
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| + | ==Improvement by USTC 2016== | ||
| + | <br> | ||
| + | Our part is Part:<html> | ||
| + | <a href="https://parts.igem.org/Part:BBa_JK2009357"> BBa_K2009357</a> | ||
| + | </html>.We construted this composite by ligating GAL1 promoter + Kozak sequence (Part:BBa_J63006) and (2) GFP (Part:BBa_E0040). However, the ATG inside the KOZAK sequence won't be in the same reading frame as the ATG of the downstream coding sequence, so a frame-shift mutation is inevitable. To solve this problem, we add a base pair after the Kozak sequence so that the ATG can be in the same reading frame and the GFP can be expressed properly. What’s more, this part become “ready to use”, which means the GFP sequence can be directly altered by other functional parts and the sequence will be expressed properly. | ||
Revision as of 01:17, 1 October 2016
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K401001
User Reviews
UNIQ7c3d0a658a5e032b-partinfo-00000000-QINU UNIQ7c3d0a658a5e032b-partinfo-00000001-QINU
Improvement by USTC 2016
Our part is Part:
BBa_K2009357
.We construted this composite by ligating GAL1 promoter + Kozak sequence (Part:BBa_J63006) and (2) GFP (Part:BBa_E0040). However, the ATG inside the KOZAK sequence won't be in the same reading frame as the ATG of the downstream coding sequence, so a frame-shift mutation is inevitable. To solve this problem, we add a base pair after the Kozak sequence so that the ATG can be in the same reading frame and the GFP can be expressed properly. What’s more, this part become “ready to use”, which means the GFP sequence can be directly altered by other functional parts and the sequence will be expressed properly.