Difference between revisions of "Part:BBa I716001:Design"

 
(→‎Design Notes)
 
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===Design Notes===
 
===Design Notes===
 +
{{BerkiGEM2007-Biobrick2.0Intro}}
 
<pre>
 
<pre>
 
Bbrick ORF neuS
 
Bbrick ORF neuS
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AY004.neus-R    gtcagCTCGAGttaGGATCCttactcccccaagaaaatcc
 
AY004.neus-R    gtcagCTCGAGttaGGATCCttactcccccaagaaaatcc
 
</pre>
 
</pre>
−
 
−
 
  
 
===Source===
 
===Source===

Latest revision as of 19:06, 6 June 2007


neuS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 100


Design Notes


This part is built in Biobrick version 2.0. In this format, parts are flanked by BglII and BamHI restriction sites. 

Bbrick ORF neuS
[pcr] neus-F/R on pJ23006-Bca9106        (1261bp, BglII/XhoI)
[sub] into pBca9145-Bca1144#5            (BglII/XhoI, 2063+910, L)
[prod] pBca9145-neuS (I716001)

---
AY003.neus-F     ctacgAGATCTatgatatttgatgctagtttaaag
AY004.neus-R     gtcagCTCGAGttaGGATCCttactcccccaagaaaatcc

Source

pJ23006-Bca9106

References