Difference between revisions of "Part:BBa K2060000"
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This part includes a guide RNA that will target Cas9 to the E.Coli 16S ribosomal RNA locus. The guide RNA is expressed with the Cas9-recognised RNA scaffold. Expression of the guideRNA is driven by the T7 promotor with Lac0 binding site and followed by a T7 terminator sequence. | This part includes a guide RNA that will target Cas9 to the E.Coli 16S ribosomal RNA locus. The guide RNA is expressed with the Cas9-recognised RNA scaffold. Expression of the guideRNA is driven by the T7 promotor with Lac0 binding site and followed by a T7 terminator sequence. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
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Latest revision as of 15:39, 30 September 2016
CRISPR-Cas9 guide RNA targeting to 16S RNA
This part includes a guide RNA that will target Cas9 to the E.Coli 16S ribosomal RNA locus. The guide RNA is expressed with the Cas9-recognised RNA scaffold. Expression of the guideRNA is driven by the T7 promotor with Lac0 binding site and followed by a T7 terminator sequence.
Usage and Biology
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 48
- 1000COMPATIBLE WITH RFC[1000]